Covaris e220 user manual

The company refers to this process as adaptive focused acoustic energy. Contact covaris customer support for further assistance. Unpacking and instructions covaris sseries youtube. Tissue chip protocols, cultured cells chip protocols, msds information. Dna shearing with microtubes covaris water conditioning system. The covaris e220 evolution instrument available at ggbc is mostly used for dna shearing application.

Covaris s220 ultrasonicator user operated holders and tubes are available for 50ul to 2ml sample volumes. Similarly, insufficient degas levels within the bath may result in poor acoustic coupling and thereby shift the mean fragment size. With over 18 years experience in consultation and a strong background in industry, we can progress your projects and provide solutions. Table 10 shear settings for covaris eseries instrument sonolab software v7 or later. Utilizing a spherical acoustic transducer, specifically designed acoustical cuvettes and advanced electronics, afa delivers controlled energy precisely and accurately to sample volumes from. Instrument and software developed for highperformance sample. The e220 can be operated as a standalone unit or easily integrated as part of an automated laboratory system. Documents document view page perkin elmer janus liquid. User manual an automated, batch system for high performance sample preparation using adaptive focused acoustic energy.

Utilizing a dishshaped acoustic transducer and computercontrolled electronics. Recommended settings are subject to change without notice. Covaris e220 focusedultrasonicator research homogenizer dnashearing cell lysis duration. Each strip contains 8 microtube and allows great flexibility when processing samples in. This manual contains operation and maintenance instructions for the covaris e and leseries instruments the e220, e220x and le220. If you are using a covaris model that is not supported, illumina recommends that you use the covaris e220 settings, found in the e220 user manual, as a starting point. Dna shearing with covaris focusedultrasonicators, dna shearing for the s220 e220, dna shearing with gtube, trushear buffer dna shearing. Cp02 automated cryoprep system user manual cp01 cryoprep manual. Covaris assumes no liability regarding the use of a protocol for applications in which it is not intended or with use of non covaris certified consumables. This manual includes sections on the operating instructions, maintenance guidelines, and troubleshooting tips. The covaris instrument works by using acoustic energy wave. The covaris m220 ultrasonicator provides mechanical shearing of dna to generate tight, highly reproducible fragment distributions. Ele220 series user manual page 4 warranty when used in accordance with written instruction and under normal operating conditions, the covaris instruments are guaranteed to be free of defects in material and workmanship for one 1 year from the date of original delivery by an authorized representative.

Consult the manufacturers recommendations for use of other covaris instruments or sample holders to achieve the same target dna fragment size. S220 x user manual page 4 warranty when used in accordance with written instruction and under normal operating conditions, the covaris instruments are guaranteed to be free of defects in material and workmanship for one 1 year from the date of original delivery by an authorized representative. Dna shearing with e220 focusedultrasonicator covaris. Optimization of covaris settings for shearing bacterial. It is ideal for the production of dna libraries for nextgeneration sequencing. I have been working at covaris fulltime more than 8 years pros. The target fragment size range can be chosen between 150 bp and 5 kb. It contains background information essential to the proper use and care of this equipment. This instrument can used by customers for precisely shearing dna in preparation for illumina sequencing and other applications. The versatility of the eseries focusedultrasonicator makes it possible to bring the advantages of afa to numerous biological and chemical applications including, dna and chromatin shearing, tissue homogenization, cell lysis. Covaris le220 focused ultrasonicator bostonind 873. Chiller for sseries, eseries and leseries afa ultrasonicators. Note this protocol has been optimized using a covaris model e220 instrument and the l covaris microtube. System degas pumps should be run in advance of and during afa treatments, as detailed in instrument user manuals.

Please note while using the e220 and e220evolution, the intensifier. See the question what are the settings for the s220 and m220 covaris models. The covaris focused sonicators are the standard equipment to reproducibly shear nucleic acid and chromatin in an unbiased fashion for highthroughput. Covaris e210 user manual uncheck hide file extensions for known file types my boss did lose a lot of money by losing the contract, said dogg. The e220 may be programed to process from 1 to 96 samples in a single batch. Set up the covaris s2 or e210 at the appropriate temperature. Each strip contains 8 microtube and allows great flexibility when processing samples in varying batch sizes. Preparation using adaptive focused acoustic afa energy. Covaris covaris e110 focused ultrasonicator covaris e220 focused ultrasonicator multisample w vwr mm7 recirculating chiller. Mechanical shearing for next generation sequencing ngs dnarna extraction from formalinfixed, paraffinembedded.

Two parameters were mod ulated to optimize the yields of gdna fragments in the size range of interest. If youre a talented person, that can get things done and can bring value to the company, then covaris is the best place to work for you. The e220 evolution and the covaris process the e220 evolution focusedultrasonicators are based on covaris adaptive focused acoustics afa technology. Covaris e220 focused ultrasonicator multisample w vwr mm7. This kit allows users to routinely verify the performance of their covaris. The e220 focusedultrasonicators are versatile instruments engineered for preanalytical sample processing by adaptive focused acoustics.

Used covaris e220 focused ultrasonicator sample preparer. Optimized shearing protocols are available for fragment sizes from 150 to 5,000 bp. Company covaris, inc price get quote get quote for this product. Covaris e210 focused ultrasonicator wchiller circulator predecessor to e220 10808 duration. This instrument is designed to provide researchers an integrated standalone tool to be used in a laboratory environment. The e220 evolution enables multisample, batch preparation, capable of processing a wide range of sample types and volumes and may be programed to process from 1 to 8 samples in a single batch. The e220 can be operated as a standalone unit, or easily integrated as part of an automated laboratory system. Autoplay when autoplay is enabled, a suggested video will automatically play next. The e220 focusedultrasonicator enables multisample batch preparation, capable of processing a wide range of sample types and volumes. Related documents document title document number raindance 3kb shearing pr. The covaris e220 enables highthroughput sample sonication and processes from one to 96 samples in a 96well plate or in individual glass microtubes. Covaris are your leading technical specialists in physical asset management, analytics, maintenance and change management.